Mutants of Sweetclover (Melilotus alba) Lacking Chlorophyll b

Mutants of Sweetclover (Melilotus alba) Lacking Chlorophyll b: Studies on Pigment-Protein Complexes and Thylakoid Protein Phosphorylation.

Mutants of sweetclover (Melilotus alba) with defects in the nuclear ch5 locus were examined. Using thin-layer chromatography and absorption spectroscopy, three of these mutants were found to lack chlorophyll (Chl) b. One of these three mutants, U374, possessed thylakoid membranes lacking the three Chl b-containing pigment-protein complexes (AB-1, AB-2, and AB-3) while still containing A-1 and A...

A Temperature-Sensitive Chlorophyll b-Deficient Mutant of Sweetclover (Melilotus alba).

The ch4 mutant of sweetclover (Melilotus alba) has previously been demonstrated to be partially deficient in chlorophyll and to have a higher ratio of chlorophyll a to b than normal plants. We were able to substantiate these findings when plants were grown at 23 degrees C and lower (permissive temperatures). However, when grown at 26 degrees C (nonpermissive temperature) the plants produced sma...

A Temperature-Sensitive Chlorophyll β-Deficient Mutant of Sweetclover (Melilotus alba)

Genetic Studies of Induced Mutants in Melilotus alba. II. Inheritance and Complementation of Chlorophyll-deficient Mutants

Six ethyl methanesulfonate-induced mutants of Melilotus alba Desr. were studied. Five of the mutants behaved as monogenic recessives. In the sixth mutant, two independent recessive alleles were responsible for the observed phenotype. Of these two genes, one had a phenotypic effect similar to the five single-gene mutants. The other (the veined gene) caused a chlorophyll deficiency in which the l...

Contents of Chlorophylls a and b in Chlorophyll-Deficient Mutants of Sweetclover

Leaf extracts from twelve chlorophyll-deficient mutants of sweetc10ver (Melilotus alba Desr_) were compared spectrophotometrically and chromatographically with extracts from normal plants to determine contents of chlorophylls a and b_ Total chlorophyll contents in cotyledons and leaves of all mutants were significantly lower than those in corresponding tissues of normal plants_ Chlorophyll cont...